Human Gene EIF2AK4 (uc001zkm.1) Description and Page Index
  Description: Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), mRNA.
RefSeq Summary (NM_001013703): This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000263791.10/ ENSP00000263791.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr15:40,226,347-40,327,797 Size: 101,451 Total Exon Count: 39 Strand: +
Coding Region
   Position: hg19 chr15:40,226,397-40,327,285 Size: 100,889 Coding Exon Count: 39 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-15

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr15:40,226,347-40,327,797)mRNA (may differ from genome)Protein (1649 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
OMIMPubMedStanford SOURCETreefamUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 2-alpha kinase 4; EC=; AltName: Full=GCN2-like protein;
FUNCTION: Can phosphorylate the alpha subunit of EIF2 and may mediate translational control (By similarity).
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
TISSUE SPECIFICITY: Widely expressed.
DOMAIN: Kinase domain 1 is a degenerate kinase domain.
DOMAIN: RWD domain is reported to interact with GCN1L1.
PTM: Autophosphorylated on threonine residues (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
SIMILARITY: Contains 2 protein kinase domains.
SIMILARITY: Contains 1 RWD domain.
SEQUENCE CAUTION: Sequence=BAA92576.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part; Sequence=BAB15625.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

-  MalaCards Disease Associations
  MalaCards Gene Search: EIF2AK4
Diseases sorted by gene-association score: pulmonary venoocclusive disease 2* (1691), pulmonary venoocclusive disease 1* (247), heritable pulmonary arterial hypertension* (157), pulmonary venoocclusive disease (35), yellow fever (32), pulmonary hypertension (7)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.52 RPKM in Cells - Cultured fibroblasts
Total median expression: 285.37 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -25.0050-0.500 Picture PostScript Text
3' UTR -157.00512-0.307 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004154 - Anticodon-bd
IPR026920 - EIF2AK4
IPR024435 - GCN2_anticodon-bd_dom
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR006575 - RWD-domain
IPR008271 - Ser/Thr_kinase_AS
IPR016255 - Ser/Thr_kinase_GCN2
IPR016135 - UBQ-conjugating_enzyme/RWD

Pfam Domains:
PF00069 - Protein kinase domain
PF05773 - RWD domain
PF07714 - Protein tyrosine kinase
PF12745 - Anticodon binding domain of tRNAs
PF13393 - Histidyl-tRNA synthetase

SCOP Domains:
52954 - Anticodon-binding domain of Class II aaRS
55681 - Class II aaRS and biotin synthetases
56112 - Protein kinase-like (PK-like)
54495 - UBC-like

ModBase Predicted Comparative 3D Structure on Q9P2K8
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserGenome BrowserGenome Browser
Gene Details  Gene DetailsGene DetailsGene Details
Gene Sorter  Gene SorterGene SorterGene Sorter
  Protein SequenceProtein SequenceProtein SequenceProtein Sequence

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0003723 RNA binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004694 eukaryotic translation initiation factor 2alpha kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0000077 DNA damage checkpoint
GO:0002230 positive regulation of defense response to virus by host
GO:0002250 adaptive immune response
GO:0002286 T cell activation involved in immune response
GO:0002376 immune system process
GO:0002821 positive regulation of adaptive immune response
GO:0006417 regulation of translation
GO:0006446 regulation of translational initiation
GO:0006468 protein phosphorylation
GO:0007049 cell cycle
GO:0007050 cell cycle arrest
GO:0007399 nervous system development
GO:0007612 learning
GO:0007616 long-term memory
GO:0010998 regulation of translational initiation by eIF2 alpha phosphorylation
GO:0016032 viral process
GO:0016310 phosphorylation
GO:0019081 viral translation
GO:0032057 negative regulation of translational initiation in response to stress
GO:0032792 negative regulation of CREB transcription factor activity
GO:0034198 cellular response to amino acid starvation
GO:0034644 cellular response to UV
GO:0036492 eiF2alpha phosphorylation in response to endoplasmic reticulum stress
GO:0039520 induction by virus of host autophagy
GO:0044828 negative regulation by host of viral genome replication
GO:0045665 negative regulation of neuron differentiation
GO:0045947 negative regulation of translational initiation
GO:0046777 protein autophosphorylation
GO:0051607 defense response to virus
GO:0060259 regulation of feeding behavior
GO:0060733 regulation of eIF2 alpha phosphorylation by amino acid starvation
GO:0070417 cellular response to cold
GO:0071264 positive regulation of translational initiation in response to starvation
GO:1900273 positive regulation of long-term synaptic potentiation
GO:1990138 neuron projection extension
GO:1990253 cellular response to leucine starvation

Cellular Component:
GO:0005737 cytoplasm
GO:0005844 polysome
GO:0022626 cytosolic ribosome

-  Descriptions from all associated GenBank mRNAs
  BC078179 - Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4, mRNA (cDNA clone IMAGE:6503617), complete cds.
BC061591 - Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4, mRNA (cDNA clone IMAGE:6504179).
LF205839 - JP 2014500723-A/13342: Polycomb-Associated Non-Coding RNAs.
KJ904295 - Synthetic construct Homo sapiens clone ccsbBroadEn_13689 EIF2AK4 gene, encodes complete protein.
AB527135 - Synthetic construct DNA, clone: pF1KB7325, Homo sapiens EIF2AK4 gene for eukaryotic translation initiation factor 2 alpha kinase 4, without stop codon, in Flexi system.
BC146319 - Synthetic construct Homo sapiens clone IMAGE:100015174, MGC:180235 eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4) mRNA, encodes complete protein.
CU687326 - Synthetic construct Homo sapiens gateway clone IMAGE:100022967 5' read EIF2AK4 mRNA.
MA441416 - JP 2018138019-A/13342: Polycomb-Associated Non-Coding RNAs.
JD405599 - Sequence 386623 from Patent EP1572962.
LF348783 - JP 2014500723-A/156286: Polycomb-Associated Non-Coding RNAs.
AB037759 - Homo sapiens mRNA for KIAA1338 protein, partial cds.
AL832907 - Homo sapiens mRNA; cDNA DKFZp762C0914 (from clone DKFZp762C0914).
LF348786 - JP 2014500723-A/156289: Polycomb-Associated Non-Coding RNAs.
BC014915 - Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4, mRNA (cDNA clone IMAGE:3920536), with apparent retained intron.
LF348789 - JP 2014500723-A/156292: Polycomb-Associated Non-Coding RNAs.
LF348791 - JP 2014500723-A/156294: Polycomb-Associated Non-Coding RNAs.
AL157497 - Homo sapiens mRNA; cDNA DKFZp434F1312 (from clone DKFZp434F1312); partial cds.
BC019914 - Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4, mRNA (cDNA clone IMAGE:4994095), with apparent retained intron.
BC009350 - Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4, mRNA (cDNA clone IMAGE:4137621), partial cds.
AK027011 - Homo sapiens cDNA: FLJ23358 fis, clone HEP14996.
KJ905671 - Synthetic construct Homo sapiens clone ccsbBroadEn_15327 EIF2AK4 gene, encodes complete protein.
CU679311 - Synthetic construct Homo sapiens gateway clone IMAGE:100020100 5' read EIF2AK4 mRNA.
KJ905109 - Synthetic construct Homo sapiens clone ccsbBroadEn_14503 EIF2AK4 gene, encodes complete protein.
BX649048 - Homo sapiens mRNA; cDNA DKFZp779G0220 (from clone DKFZp779G0220).
LF348796 - JP 2014500723-A/156299: Polycomb-Associated Non-Coding RNAs.
LF348798 - JP 2014500723-A/156301: Polycomb-Associated Non-Coding RNAs.
LF348799 - JP 2014500723-A/156302: Polycomb-Associated Non-Coding RNAs.
AK074361 - Homo sapiens cDNA FLJ23781 fis, clone HEP20945, highly similar to Probable translation initiation factor eIF-2alpha kinase (EC 2.7.1.-).
AJ243428 - Homo sapiens partial mRNA for putative eIF2 alpha kinase (GCN2 gene).
AL137676 - Homo sapiens mRNA; cDNA DKFZp434H149 (from clone DKFZp434H149).
LF348800 - JP 2014500723-A/156303: Polycomb-Associated Non-Coding RNAs.
AL137627 - Homo sapiens mRNA; cDNA DKFZp434P0612 (from clone DKFZp434P0612); partial cds.
LF348801 - JP 2014500723-A/156304: Polycomb-Associated Non-Coding RNAs.
JD258075 - Sequence 239099 from Patent EP1572962.
JD236711 - Sequence 217735 from Patent EP1572962.
JD536175 - Sequence 517199 from Patent EP1572962.
JD376010 - Sequence 357034 from Patent EP1572962.
JD497684 - Sequence 478708 from Patent EP1572962.
JD542600 - Sequence 523624 from Patent EP1572962.
JD212472 - Sequence 193496 from Patent EP1572962.
JD212474 - Sequence 193498 from Patent EP1572962.
JD212473 - Sequence 193497 from Patent EP1572962.
JD197857 - Sequence 178881 from Patent EP1572962.
JD197856 - Sequence 178880 from Patent EP1572962.
JD412880 - Sequence 393904 from Patent EP1572962.
JD412882 - Sequence 393906 from Patent EP1572962.
JD412881 - Sequence 393905 from Patent EP1572962.
JD512866 - Sequence 493890 from Patent EP1572962.
JD512868 - Sequence 493892 from Patent EP1572962.
JD512867 - Sequence 493891 from Patent EP1572962.
JD468724 - Sequence 449748 from Patent EP1572962.
JD436786 - Sequence 417810 from Patent EP1572962.
JD074827 - Sequence 55851 from Patent EP1572962.
JD424469 - Sequence 405493 from Patent EP1572962.
JD052757 - Sequence 33781 from Patent EP1572962.
JD136149 - Sequence 117173 from Patent EP1572962.
JD243193 - Sequence 224217 from Patent EP1572962.
JD079516 - Sequence 60540 from Patent EP1572962.
JD180198 - Sequence 161222 from Patent EP1572962.
JD449728 - Sequence 430752 from Patent EP1572962.
JD249288 - Sequence 230312 from Patent EP1572962.
JD376926 - Sequence 357950 from Patent EP1572962.
JD353457 - Sequence 334481 from Patent EP1572962.
JD260336 - Sequence 241360 from Patent EP1572962.
JD421150 - Sequence 402174 from Patent EP1572962.
JD532432 - Sequence 513456 from Patent EP1572962.
JD443617 - Sequence 424641 from Patent EP1572962.
JD147522 - Sequence 128546 from Patent EP1572962.
JD445121 - Sequence 426145 from Patent EP1572962.
JD444658 - Sequence 425682 from Patent EP1572962.
JD548960 - Sequence 529984 from Patent EP1572962.
JD495653 - Sequence 476677 from Patent EP1572962.
JD265395 - Sequence 246419 from Patent EP1572962.
JD174543 - Sequence 155567 from Patent EP1572962.
JD060131 - Sequence 41155 from Patent EP1572962.
JD423113 - Sequence 404137 from Patent EP1572962.
JD537901 - Sequence 518925 from Patent EP1572962.
JD461329 - Sequence 442353 from Patent EP1572962.
JD518764 - Sequence 499788 from Patent EP1572962.
JD385110 - Sequence 366134 from Patent EP1572962.
JD375451 - Sequence 356475 from Patent EP1572962.
JD160104 - Sequence 141128 from Patent EP1572962.
MA584360 - JP 2018138019-A/156286: Polycomb-Associated Non-Coding RNAs.
MA584363 - JP 2018138019-A/156289: Polycomb-Associated Non-Coding RNAs.
MA584366 - JP 2018138019-A/156292: Polycomb-Associated Non-Coding RNAs.
MA584368 - JP 2018138019-A/156294: Polycomb-Associated Non-Coding RNAs.
MA584373 - JP 2018138019-A/156299: Polycomb-Associated Non-Coding RNAs.
MA584375 - JP 2018138019-A/156301: Polycomb-Associated Non-Coding RNAs.
MA584376 - JP 2018138019-A/156302: Polycomb-Associated Non-Coding RNAs.
MA584377 - JP 2018138019-A/156303: Polycomb-Associated Non-Coding RNAs.
MA584378 - JP 2018138019-A/156304: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_eif2Pathway - Regulation of eIF2

-  Other Names for This Gene
  Alternate Gene Symbols: C9JEC4, E2AK4_HUMAN, GCN2, KIAA1338, NM_001013703, NP_001013725, Q69YL7, Q6DC97, Q96GN6, Q9H5K1, Q9NSQ3, Q9NSZ5, Q9P2K8, Q9UJ56
UCSC ID: uc001zkm.1
RefSeq Accession: NM_001013703
Protein: Q9P2K8 (aka E2AK4_HUMAN)
CCDS: CCDS42016.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_001013703.2
exon count: 39CDS single in 3' UTR: no RNA size: 5532
ORF size: 4950CDS single in intron: no Alignment % ID: 99.98
txCdsPredict score: 9977.00frame shift in genome: no % Coverage: 99.64
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.