Human Gene ST3GAL2 (uc002eyx.2) Description and Page Index
  Description: Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2), mRNA.
RefSeq Summary (NM_006927): The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A. [provided by RefSeq, Jul 2008]. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.40910.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000342907.3/ ENSP00000345477.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr16:70,413,338-70,472,991 Size: 59,654 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr16:70,415,592-70,432,433 Size: 16,842 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-15

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:70,413,338-70,472,991)mRNA (may differ from genome)Protein (350 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedReactomeStanford SOURCETreefamUniProtKB

-  Comments and Description Text from UniProtKB
  ID: SIA4B_HUMAN
DESCRIPTION: RecName: Full=CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2; Short=Alpha 2,3-ST 2; Short=Beta-galactoside alpha-2,3-sialyltransferase 2; EC=2.4.99.4; AltName: Full=Gal-NAc6S; AltName: Full=Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; AltName: Full=ST3Gal II; Short=ST3GalII; AltName: Full=ST3GalA.2; AltName: Full=Sialyltransferase 4B; Short=SIAT4-B;
FUNCTION: It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found in terminal carbohydrate groups of certain glycoproteins, oligosaccharides and glycolipids. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.
CATALYTIC ACTIVITY: CMP-N-acetylneuraminate + beta-D-galactosyl- 1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N- acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D- galactosaminyl-R.
PATHWAY: Protein modification; protein glycosylation.
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein (By similarity). Secreted (By similarity). Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid (By similarity).
TISSUE SPECIFICITY: Highly expressed in skeletal muscle and heart and to a much lesser extent in brain, placenta, liver and pancreas. Scarcely detectable in lung and kidney.
PTM: The soluble form derives from the membrane form by proteolytic processing (By similarity).
SIMILARITY: Belongs to the glycosyltransferase 29 family.
WEB RESOURCE: Name=GGDB; Note=GlycoGene database; URL="http://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=ST3GAL2";
WEB RESOURCE: Name=Functional Glycomics Gateway - GTase; Note=ST3Gal II; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_623";

-  MalaCards Disease Associations
  MalaCards Gene Search: ST3GAL2
Diseases sorted by gene-association score: kidney fibrosarcoma (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 18.42 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 250.53 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -542.001143-0.474 Picture PostScript Text
3' UTR -957.932254-0.425 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001675 - Glyco_trans_29
IPR012163 - Sialyl_trans

Pfam Domains:
PF00777 - Glycosyltransferase family 29 (sialyltransferase)

ModBase Predicted Comparative 3D Structure on Q16842
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
 Protein SequenceProtein Sequence   
 AlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003836 beta-galactoside (CMP) alpha-2,3-sialyltransferase activity
GO:0008373 sialyltransferase activity
GO:0016740 transferase activity
GO:0016757 transferase activity, transferring glycosyl groups
GO:0047288 monosialoganglioside sialyltransferase activity

Biological Process:
GO:0006486 protein glycosylation
GO:0009101 glycoprotein biosynthetic process
GO:0009247 glycolipid biosynthetic process
GO:0009311 oligosaccharide metabolic process
GO:0009312 oligosaccharide biosynthetic process
GO:0016266 O-glycan processing
GO:0018146 keratan sulfate biosynthetic process
GO:0018279 protein N-linked glycosylation via asparagine
GO:0030259 lipid glycosylation
GO:0097503 sialylation
GO:1990743 protein sialylation

Cellular Component:
GO:0000139 Golgi membrane
GO:0005576 extracellular region
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0032580 Golgi cisterna membrane


-  Descriptions from all associated GenBank mRNAs
  JD127876 - Sequence 108900 from Patent EP1572962.
JD056118 - Sequence 37142 from Patent EP1572962.
JD306607 - Sequence 287631 from Patent EP1572962.
JD169927 - Sequence 150951 from Patent EP1572962.
JD529556 - Sequence 510580 from Patent EP1572962.
JD430387 - Sequence 411411 from Patent EP1572962.
JD118360 - Sequence 99384 from Patent EP1572962.
JD118359 - Sequence 99383 from Patent EP1572962.
JD437105 - Sequence 418129 from Patent EP1572962.
JD421472 - Sequence 402496 from Patent EP1572962.
JD559915 - Sequence 540939 from Patent EP1572962.
JD199464 - Sequence 180488 from Patent EP1572962.
JD085887 - Sequence 66911 from Patent EP1572962.
JD047711 - Sequence 28735 from Patent EP1572962.
JD363145 - Sequence 344169 from Patent EP1572962.
JD238410 - Sequence 219434 from Patent EP1572962.
JD047712 - Sequence 28736 from Patent EP1572962.
JD238411 - Sequence 219435 from Patent EP1572962.
JD135865 - Sequence 116889 from Patent EP1572962.
JD135866 - Sequence 116890 from Patent EP1572962.
JD100548 - Sequence 81572 from Patent EP1572962.
JD173454 - Sequence 154478 from Patent EP1572962.
JD378228 - Sequence 359252 from Patent EP1572962.
JD039711 - Sequence 20735 from Patent EP1572962.
JD473857 - Sequence 454881 from Patent EP1572962.
JD139888 - Sequence 120912 from Patent EP1572962.
JD168687 - Sequence 149711 from Patent EP1572962.
JD483003 - Sequence 464027 from Patent EP1572962.
JD461079 - Sequence 442103 from Patent EP1572962.
JD537831 - Sequence 518855 from Patent EP1572962.
JD222202 - Sequence 203226 from Patent EP1572962.
JD100952 - Sequence 81976 from Patent EP1572962.
JD404899 - Sequence 385923 from Patent EP1572962.
BC008779 - Homo sapiens cDNA clone IMAGE:3607101, **** WARNING: chimeric clone ****.
BC019001 - Homo sapiens cDNA clone IMAGE:3613805, **** WARNING: chimeric clone ****.
AF086257 - Homo sapiens full length insert cDNA clone ZD41E06.
BC064480 - Homo sapiens cDNA clone IMAGE:5093665, partial cds.
AK127322 - Homo sapiens cDNA FLJ45393 fis, clone BRHIP3027105, highly similar to CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 3-sialyltransferase (EC 2.4.99.-).
JD512843 - Sequence 493867 from Patent EP1572962.
JD412863 - Sequence 393887 from Patent EP1572962.
JD197849 - Sequence 178873 from Patent EP1572962.
JD212458 - Sequence 193482 from Patent EP1572962.
JD512842 - Sequence 493866 from Patent EP1572962.
JD196111 - Sequence 177135 from Patent EP1572962.
JD058038 - Sequence 39062 from Patent EP1572962.
JD412862 - Sequence 393886 from Patent EP1572962.
JD538335 - Sequence 519359 from Patent EP1572962.
JD240445 - Sequence 221469 from Patent EP1572962.
JD503049 - Sequence 484073 from Patent EP1572962.
JD231405 - Sequence 212429 from Patent EP1572962.
JD549534 - Sequence 530558 from Patent EP1572962.
JD387877 - Sequence 368901 from Patent EP1572962.
JD536675 - Sequence 517699 from Patent EP1572962.
JD193923 - Sequence 174947 from Patent EP1572962.
JD406412 - Sequence 387436 from Patent EP1572962.
JD128327 - Sequence 109351 from Patent EP1572962.
JD522847 - Sequence 503871 from Patent EP1572962.
JD197709 - Sequence 178733 from Patent EP1572962.
BC036777 - Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2, mRNA (cDNA clone MGC:46129 IMAGE:5750330), complete cds.
X96667 - H.sapiens mRNA for Gal beta-1,3 GalNAc alpha-2,3-sialyltransferase.
JD163870 - Sequence 144894 from Patent EP1572962.
JD363069 - Sequence 344093 from Patent EP1572962.
JD405051 - Sequence 386075 from Patent EP1572962.
U63090 - Human Gal beta-1,3 GalNAc alpha-2,3 sialyltransferase (ST3Gal II) mRNA, complete cds.
JD439451 - Sequence 420475 from Patent EP1572962.
JD520554 - Sequence 501578 from Patent EP1572962.
AK313020 - Homo sapiens cDNA, FLJ93488, highly similar to Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2), mRNA.
JF432706 - Synthetic construct Homo sapiens clone IMAGE:100073945 ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) gene, encodes complete protein.
KJ892139 - Synthetic construct Homo sapiens clone ccsbBroadEn_01533 ST3GAL2 gene, encodes complete protein.
AB529058 - Synthetic construct DNA, clone: pF1KB3774, Homo sapiens ST3GAL2 gene for ST3 beta-galactoside alpha-2,3-sialyltransferase 2, without stop codon, in Flexi system.
JD143019 - Sequence 124043 from Patent EP1572962.
JD107695 - Sequence 88719 from Patent EP1572962.
JD215115 - Sequence 196139 from Patent EP1572962.
JD342955 - Sequence 323979 from Patent EP1572962.
JD108976 - Sequence 90000 from Patent EP1572962.
JD065727 - Sequence 46751 from Patent EP1572962.
JD131447 - Sequence 112471 from Patent EP1572962.
JD047478 - Sequence 28502 from Patent EP1572962.
JD218637 - Sequence 199661 from Patent EP1572962.
JD205896 - Sequence 186920 from Patent EP1572962.
JD391863 - Sequence 372887 from Patent EP1572962.
JD218784 - Sequence 199808 from Patent EP1572962.
JD044584 - Sequence 25608 from Patent EP1572962.
JD115576 - Sequence 96600 from Patent EP1572962.
JD040008 - Sequence 21032 from Patent EP1572962.
JD389951 - Sequence 370975 from Patent EP1572962.
JD439429 - Sequence 420453 from Patent EP1572962.
JD151838 - Sequence 132862 from Patent EP1572962.
JD077200 - Sequence 58224 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00512 - O-Glycan biosynthesis
hsa00533 - Glycosaminoglycan biosynthesis - keratan sulfate
hsa00603 - Glycosphingolipid biosynthesis - globo series
hsa00604 - Glycosphingolipid biosynthesis - ganglio series
hsa01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein Q16842 (Reactome details) participates in the following event(s):

R-HSA-981497 ST3GAL1-4 can add a sialic acid to the T antigen at the alpha 3 position
R-HSA-2046285 The keratan chain can be capped by N-acetylneuraminic acid
R-HSA-4084984 ST3GAL1-6 transfer Neu5Ac to terminal Gal (alpha-2,3 link)
R-HSA-977068 Termination of O-glycan biosynthesis
R-HSA-2022854 Keratan sulfate biosynthesis
R-HSA-4085001 Sialic acid metabolism
R-HSA-913709 O-linked glycosylation of mucins
R-HSA-1638074 Keratan sulfate/keratin metabolism
R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
R-HSA-5173105 O-linked glycosylation
R-HSA-1630316 Glycosaminoglycan metabolism
R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
R-HSA-597592 Post-translational protein modification
R-HSA-71387 Metabolism of carbohydrates
R-HSA-446203 Asparagine N-linked glycosylation
R-HSA-392499 Metabolism of proteins
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: NM_006927, NP_008858, O00654, Q16842, SIA4B_HUMAN, SIAT4B
UCSC ID: uc002eyx.2
RefSeq Accession: NM_006927
Protein: Q16842 (aka SIA4B_HUMAN or SI4B_HUMAN)
CCDS: CCDS10890.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_006927.3
exon count: 7CDS single in 3' UTR: no RNA size: 4450
ORF size: 1053CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1690.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.