Human Gene NEK6 (uc004bog.3) Description and Page Index
  Description: Homo sapiens NIMA-related kinase 6 (NEK6), transcript variant 2, mRNA.
RefSeq Summary (NM_014397): The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
Transcript (Including UTRs)
   Position: hg19 chr9:127,020,196-127,114,719 Size: 94,524 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr9:127,064,244-127,113,226 Size: 48,983 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-15

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:127,020,196-127,114,719)mRNA (may differ from genome)Protein (313 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
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H-INVHGNCLynxMGIneXtProtOMIM
PubMedReactomeStanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: NEK6_HUMAN
DESCRIPTION: RecName: Full=Serine/threonine-protein kinase Nek6; EC=2.7.11.1; AltName: Full=Never in mitosis A-related kinase 6; Short=NimA-related protein kinase 6; AltName: Full=Protein kinase SID6-1512;
FUNCTION: Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108.
SUBUNIT: Interacts with STAT3 and RPS6KB1 (By similarity). Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4.
INTERACTION: Q13526:PIN1; NbExp=3; IntAct=EBI-740364, EBI-714158;
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Co-localizes with APBB1 at the nuclear speckles. Co-localizes with PIN1 in the nucleus. Co-localizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis.
TISSUE SPECIFICITY: Ubiquitous, with highest expression in heart and skeletal muscle. Up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers.
INDUCTION: Up-regulated during the M phase of cell cycle progression. Down-regulated in both replicative and premature senescence of cancer cells.
DOMAIN: Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.
PTM: Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.
SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=AAG13417.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part; Sequence=AAH00101.2; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH04174.2; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH04209.2; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAA85045.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): NEK6
CDC HuGE Published Literature: NEK6
Positive Disease Associations: Body Height , Cognitive performance
Related Studies:
  1. Body Height
    , , . [PubMed 0]
  2. Body Height
    , , . [PubMed 0]
  3. Body Height
    , , . [PubMed 0]
           more ... click here to view the complete list

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D001564 Benzo(a)pyrene
  • C006253 pirinixic acid
  • C016403 2,4-dinitrotoluene
  • C532162 2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine
  • D015073 2-Acetylaminofluorene
  • C035207 4-amino-2,6-dinitrotoluene
  • C027576 4-hydroxy-2-nonenal
  • D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
  • D015124 8-Bromo Cyclic Adenosine Monophosphate
  • D000082 Acetaminophen
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 62.25 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 376.43 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -123.00198-0.621 Picture PostScript Text
3' UTR -485.251493-0.325 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR001245 - Ser-Thr/Tyr_kinase_cat_dom
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q9HC98
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0001222 transcription corepressor binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0019894 kinesin binding
GO:0019901 protein kinase binding
GO:0031625 ubiquitin protein ligase binding
GO:0033613 activating transcription factor binding
GO:0046872 metal ion binding

Biological Process:
GO:0006468 protein phosphorylation
GO:0006915 apoptotic process
GO:0007049 cell cycle
GO:0007059 chromosome segregation
GO:0007077 mitotic nuclear envelope disassembly
GO:0007346 regulation of mitotic cell cycle
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0030071 regulation of mitotic metaphase/anaphase transition
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0046777 protein autophosphorylation
GO:0051225 spindle assembly
GO:0051301 cell division
GO:2000772 regulation of cellular senescence

Cellular Component:
GO:0000922 spindle pole
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005815 microtubule organizing center
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0016607 nuclear speck
GO:0032991 macromolecular complex
GO:0043231 intracellular membrane-bounded organelle
GO:0005813 centrosome


-  Descriptions from all associated GenBank mRNAs
  BC000101 - Homo sapiens NIMA (never in mitosis gene a)-related kinase 6, mRNA (cDNA clone MGC:1205 IMAGE:3507262), complete cds.
BC004174 - Homo sapiens NIMA (never in mitosis gene a)-related kinase 6, mRNA (cDNA clone MGC:2768 IMAGE:2958695), complete cds.
BC004209 - Homo sapiens NIMA (never in mitosis gene a)-related kinase 6, mRNA (cDNA clone MGC:4434 IMAGE:2958695), complete cds.
BC012761 - Homo sapiens NIMA (never in mitosis gene a)-related kinase 6, mRNA (cDNA clone MGC:16199 IMAGE:3640103), complete cds.
AB026289 - Homo sapiens mRNA for protein kinase SID6-1512, complete cds.
AF087909 - Homo sapiens NIMA-related kinase 6 (NEK6) mRNA, complete cds.
AK313071 - Homo sapiens cDNA, FLJ93552, Homo sapiens NIMA (never in mitosis gene a)-related kinase 6 (NEK6), mRNA.
AK294614 - Homo sapiens cDNA FLJ55200 complete cds, highly similar to Serine/threonine-protein kinase Nek6 (EC 2.7.11.1).
AB384987 - Synthetic construct DNA, clone: pF1KB4782, Homo sapiens NEK6 gene for serine/threonine-protein kinase Nek6, complete cds, without stop codon, in Flexi system.
JD392026 - Sequence 373050 from Patent EP1572962.
CU674034 - Synthetic construct Homo sapiens gateway clone IMAGE:100018847 5' read NEK6 mRNA.
KJ898286 - Synthetic construct Homo sapiens clone ccsbBroadEn_07680 NEK6 gene, encodes complete protein.
KJ905404 - Synthetic construct Homo sapiens clone ccsbBroadEn_14978 NEK6 gene, encodes complete protein.
CR457091 - Homo sapiens full open reading frame cDNA clone RZPDo834E1011D for gene NEK6, NIMA (never in mitosis gene a)-related kinase 6; complete cds, incl. stopcodon.
CR542222 - Homo sapiens full open reading frame cDNA clone RZPDo834C0925D for gene NEK6, NIMA (never in mitosis gene a)-related kinase 6; complete cds, without stopcodon.
JD360907 - Sequence 341931 from Patent EP1572962.
JD344568 - Sequence 325592 from Patent EP1572962.
JD332912 - Sequence 313936 from Patent EP1572962.
JD449507 - Sequence 430531 from Patent EP1572962.
JD354646 - Sequence 335670 from Patent EP1572962.
JD421769 - Sequence 402793 from Patent EP1572962.
JD538538 - Sequence 519562 from Patent EP1572962.
JD175076 - Sequence 156100 from Patent EP1572962.
JD268605 - Sequence 249629 from Patent EP1572962.
JD232238 - Sequence 213262 from Patent EP1572962.
JD088111 - Sequence 69135 from Patent EP1572962.
JD442306 - Sequence 423330 from Patent EP1572962.
JD159125 - Sequence 140149 from Patent EP1572962.
JD074327 - Sequence 55351 from Patent EP1572962.
JD454734 - Sequence 435758 from Patent EP1572962.
JD214525 - Sequence 195549 from Patent EP1572962.
JD372999 - Sequence 354023 from Patent EP1572962.
JD205607 - Sequence 186631 from Patent EP1572962.
JD334829 - Sequence 315853 from Patent EP1572962.
AJ420460 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1636598.
JD251640 - Sequence 232664 from Patent EP1572962.
JD049234 - Sequence 30258 from Patent EP1572962.
JD561959 - Sequence 542983 from Patent EP1572962.
JD492302 - Sequence 473326 from Patent EP1572962.
JD064865 - Sequence 45889 from Patent EP1572962.
JD268310 - Sequence 249334 from Patent EP1572962.
JD254560 - Sequence 235584 from Patent EP1572962.
JD193788 - Sequence 174812 from Patent EP1572962.
JD319902 - Sequence 300926 from Patent EP1572962.
JD365491 - Sequence 346515 from Patent EP1572962.
JD425171 - Sequence 406195 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9HC98 (Reactome details) participates in the following event(s):

R-HSA-2980720 NEK9 binds NEK6/NEK7 in the cytosol
R-HSA-2984258 NEK9 phosphorylates NEK6/NEK7
R-HSA-2990880 NEK6/NEK7 phosphorylates NUP98
R-HSA-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7
R-HSA-2980766 Nuclear Envelope Breakdown
R-HSA-68875 Mitotic Prophase
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
R-HSA-68886 M Phase
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-1640170 Cell Cycle

-  Other Names for This Gene
  Alternate Gene Symbols: B7Z2D9, NEK6_HUMAN, NM_014397, NP_055212, Q5TBG3, Q5TBG9, Q6FG86, Q6IAR3, Q96E83, Q9HC98, Q9ULX2
UCSC ID: uc004bog.3
RefSeq Accession: NM_014397
Protein: Q9HC98 (aka NEK6_HUMAN)
CCDS: CCDS6854.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_014397.5
exon count: 10CDS single in 3' UTR: no RNA size: 2645
ORF size: 942CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1684.00frame shift in genome: no % Coverage: 99.55
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.