Human Gene PDE8A (uc010bnc.3) Description and Page Index
Description: Homo sapiens phosphodiesterase 8A (PDE8A), transcript variant 1, mRNA. RefSeq Summary (NM_002605): The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]. Transcript (Including UTRs) Position: hg19 chr15:85,525,205-85,682,376 Size: 157,172 Total Exon Count: 23 Strand: + Coding Region Position: hg19 chr15:85,634,302-85,681,134 Size: 46,833 Coding Exon Count: 15
ID:PDE8A_HUMAN DESCRIPTION: RecName: Full=High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A; EC=22.214.171.124; FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. CATALYTIC ACTIVITY: Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate. COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. ENZYME REGULATION: Inhibited by dipyridimole. Insensitive to selective PDE inhibitors including rolipram and zaprinast as well as to the non-selective inhibitor, IBMX. Unaffected by cGMP. PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. TISSUE SPECIFICITY: Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon. DOMAIN: Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. SIMILARITY: Contains 1 PAC (PAS-associated C-terminal) domain. SIMILARITY: Contains 1 PAS (PER-ARNT-SIM) domain. SEQUENCE CAUTION: Sequence=AAL18612.1; Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=AAL18613.1; Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=AAL18614.1; Type=Erroneous translation; Note=Wrong choice of CDS; Sequence=BAG54458.1; Type=Miscellaneous discrepancy; Note=Intron retention; Sequence=EAX01967.1; Type=Erroneous gene model prediction;
Genetic Association Studies of Complex Diseases and Disorders
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O60658
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0001934 positive regulation of protein phosphorylation GO:0006198 cAMP catabolic process GO:0006355 regulation of transcription, DNA-templated GO:0007165 signal transduction GO:0007186 G-protein coupled receptor signaling pathway GO:0009187 cyclic nucleotide metabolic process GO:0060548 negative regulation of cell death GO:0070374 positive regulation of ERK1 and ERK2 cascade GO:0071364 cellular response to epidermal growth factor stimulus GO:1903206 negative regulation of hydrogen peroxide-induced cell death