Human Gene ANAPC16 (uc021psp.1) Description and Page Index
  Description: Homo sapiens anaphase promoting complex subunit 16 (ANAPC16), transcript variant 3, mRNA.
Transcript (Including UTRs)
   Position: hg19 chr10:73,975,758-73,995,618 Size: 19,861 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr10:73,983,673-73,992,874 Size: 9,202 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-15

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:73,975,758-73,995,618)mRNA (may differ from genome)Protein (110 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
H-INVHGNCLynxMGIneXtProtOMIM
PubMedReactomeStanford SOURCETreefamUniProtKB

-  Comments and Description Text from UniProtKB
  ID: APC16_HUMAN
DESCRIPTION: RecName: Full=Anaphase-promoting complex subunit 16; Short=APC16; AltName: Full=Cyclosome subunit 16;
FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: The APC/C is composed of at least 12 subunits. ANAPC16 associates with the rest of the complex independently of ANAPC2 and ANAPC11.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
SIMILARITY: Belongs to the APC16 family.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 36.69 RPKM in Artery - Tibial
Total median expression: 1224.91 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -36.20100-0.362 Picture PostScript Text
3' UTR -1127.862744-0.411 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF17256 - Anaphase Promoting Complex Subunit 16

ModBase Predicted Comparative 3D Structure on Q96DE5
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
  Ensembl   
  Protein Sequence   
  Alignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Biological Process:
GO:0007049 cell cycle
GO:0016567 protein ubiquitination
GO:0031145 anaphase-promoting complex-dependent catabolic process
GO:0051301 cell division

Cellular Component:
GO:0000775 chromosome, centromeric region
GO:0000776 kinetochore
GO:0000777 condensed chromosome kinetochore
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005680 anaphase-promoting complex
GO:0005694 chromosome
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK091047 - Homo sapiens cDNA FLJ33728 fis, clone BRAWH2017523.
AX746749 - Sequence 274 from Patent EP1308459.
LF208756 - JP 2014500723-A/16259: Polycomb-Associated Non-Coding RNAs.
JD026566 - Sequence 7590 from Patent EP1572962.
EU552924 - Homo sapiens metabolic syndrome-associated protein mRNA, complete cds.
BC009530 - Homo sapiens chromosome 10 open reading frame 104, mRNA (cDNA clone MGC:10049 IMAGE:3890955), complete cds.
JD266815 - Sequence 247839 from Patent EP1572962.
JD033973 - Sequence 14997 from Patent EP1572962.
JD442419 - Sequence 423443 from Patent EP1572962.
JD203101 - Sequence 184125 from Patent EP1572962.
CU679385 - Synthetic construct Homo sapiens gateway clone IMAGE:100016672 5' read C10orf104 mRNA.
KJ895343 - Synthetic construct Homo sapiens clone ccsbBroadEn_04737 ANAPC16 gene, encodes complete protein.
JD027578 - Sequence 8602 from Patent EP1572962.
JD034335 - Sequence 15359 from Patent EP1572962.
JD234867 - Sequence 215891 from Patent EP1572962.
JD487021 - Sequence 468045 from Patent EP1572962.
LF322519 - JP 2014500723-A/130022: Polycomb-Associated Non-Coding RNAs.
JD297029 - Sequence 278053 from Patent EP1572962.
JD040944 - Sequence 21968 from Patent EP1572962.
LF322518 - JP 2014500723-A/130021: Polycomb-Associated Non-Coding RNAs.
AK023981 - Homo sapiens cDNA FLJ13919 fis, clone Y79AA1000410.
JD039555 - Sequence 20579 from Patent EP1572962.
LF322517 - JP 2014500723-A/130020: Polycomb-Associated Non-Coding RNAs.
JD435774 - Sequence 416798 from Patent EP1572962.
JD513998 - Sequence 495022 from Patent EP1572962.
JD513999 - Sequence 495023 from Patent EP1572962.
JD069821 - Sequence 50845 from Patent EP1572962.
JD415095 - Sequence 396119 from Patent EP1572962.
JD415097 - Sequence 396121 from Patent EP1572962.
JD272956 - Sequence 253980 from Patent EP1572962.
JD356416 - Sequence 337440 from Patent EP1572962.
JD341375 - Sequence 322399 from Patent EP1572962.
LF322516 - JP 2014500723-A/130019: Polycomb-Associated Non-Coding RNAs.
LF322515 - JP 2014500723-A/130018: Polycomb-Associated Non-Coding RNAs.
JD204596 - Sequence 185620 from Patent EP1572962.
JD063167 - Sequence 44191 from Patent EP1572962.
JD542757 - Sequence 523781 from Patent EP1572962.
JD212734 - Sequence 193758 from Patent EP1572962.
JD198127 - Sequence 179151 from Patent EP1572962.
JD413430 - Sequence 394454 from Patent EP1572962.
JD246993 - Sequence 228017 from Patent EP1572962.
JD246994 - Sequence 228018 from Patent EP1572962.
MA558096 - JP 2018138019-A/130022: Polycomb-Associated Non-Coding RNAs.
MA558095 - JP 2018138019-A/130021: Polycomb-Associated Non-Coding RNAs.
MA558094 - JP 2018138019-A/130020: Polycomb-Associated Non-Coding RNAs.
MA558093 - JP 2018138019-A/130019: Polycomb-Associated Non-Coding RNAs.
MA558092 - JP 2018138019-A/130018: Polycomb-Associated Non-Coding RNAs.
MA444333 - JP 2018138019-A/16259: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q96DE5 (Reactome details) participates in the following event(s):

R-HSA-174119 Free APC/C phosphorylated by Plk1
R-HSA-174132 Free APC/C phosphorylated by Cyclin B:Cdc2
R-HSA-174139 Dissociation of phospho-Cdh1 from the APC/C complex
R-HSA-141423 Binding of the MCC complex to the APC/C complex
R-HSA-174070 Association of Cdh1 with the APC/C
R-HSA-174224 Dissociation of Cdc20 from APC/C complex
R-HSA-174057 Multiubiquitination of APC/C-associated Cdh1
R-HSA-188371 Association of Cyclin A:Cdk2 with Cdh1
R-HSA-3788708 CDKN1A (p21) prevents association of Cyclin A:Cdk2 with Cdh1
R-HSA-3788725 Cdh1:APC/C complex binds EHMT1:EHMT2
R-HSA-174171 Association of Cyclin A with the APC/C
R-HSA-174238 Activation of APC/C:Cdc20 by dissociation of Cdc20:phospho-APC/C from Cdc20:phospho-APC/C:Mad2:Bub3:BubR1
R-HSA-179410 Association of Nek2A with MCC:APC/C
R-HSA-174120 Association of Cyclin B:Cdc2 with Cdc20:APC/C complex
R-HSA-174121 Association of Securin with Cdc20:APC/C complex
R-HSA-174088 Association of cell cycle proteins with the APC/C:Cdh1 complex
R-HSA-174079 Phosphorylation of Cdh1 by Cyclin A:Cdk2
R-HSA-3788705 CDKN1A (p21) prevents phosphorylation of Cdh1 by Cyclin A:Cdk2
R-HSA-3788724 Cdh1:APC/C ubiquitinates EHMT1 and EHMT2
R-HSA-174104 Ubiquitination of Cyclin A by APC/C:Cdc20 complex
R-HSA-179417 Multiubiquitination of Nek2A
R-HSA-174227 Ubiquitination of Cyclin B by phospho-APC/C:Cdc20 complex
R-HSA-174144 Ubiquitination of Securin by phospho-APC/C:Cdc20 complex
R-HSA-174195 Ubiquitination of cell cycle proteins targeted by the APC/C:Cdh1complex
R-HSA-69015 Cytoplasmic phosphorylated Cdc6 is ubiquitinated by the anaphase-promoting complex
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-HSA-176814 Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-174143 APC/C-mediated degradation of cell cycle proteins
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
R-HSA-2559583 Cellular Senescence
R-HSA-453276 Regulation of mitotic cell cycle
R-HSA-179419 APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint
R-HSA-68882 Mitotic Anaphase
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69618 Mitotic Spindle Checkpoint
R-HSA-2262752 Cellular responses to stress
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-69052 Switching of origins to a post-replicative state
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-1640170 Cell Cycle
R-HSA-68886 M Phase
R-HSA-69239 Synthesis of DNA
R-HSA-69242 S Phase
R-HSA-69306 DNA Replication

-  Other Names for This Gene
  Alternate Gene Symbols: APC16_HUMAN, C10orf104, NM_001242547, NP_775744, Q96DE5
UCSC ID: uc021psp.1
RefSeq Accession: NM_001242547
Protein: Q96DE5 (aka APC16_HUMAN)
CCDS: CCDS7314.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001242547.1
exon count: 4CDS single in 3' UTR: no RNA size: 3186
ORF size: 333CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 866.00frame shift in genome: no % Coverage: 99.72
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.