Human Gene TMPRSS2 (ENST00000678348.1) from GENCODE V44
  Description: Cell membrane; Single-pass type II membrane protein. (from UniProt O15393)
RefSeq Summary (NM_005656): This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2020].
Gencode Transcript: ENST00000678348.1
Gencode Gene: ENSG00000184012.14
Transcript (Including UTRs)
   Position: hg38 chr21:41,464,305-41,508,157 Size: 43,853 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg38 chr21:41,466,142-41,498,133 Size: 31,992 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-08-18 16:09:47

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr21:41,464,305-41,508,157)mRNA (may differ from genome)Protein (492 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Transmembrane protease serine 2; EC=3.4.21.-; AltName: Full=Serine protease 10; Contains: RecName: Full=Transmembrane protease serine 2 non-catalytic chain; Contains: RecName: Full=Transmembrane protease serine 2 catalytic chain; Flags: Precursor;
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein.
SUBCELLULAR LOCATION: Transmembrane protease serine 2 catalytic chain: Secreted. Note=Activated by cleavage and secreted.
TISSUE SPECIFICITY: Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
SIMILARITY: Belongs to the peptidase S1 family.
SIMILARITY: Contains 1 LDL-receptor class A domain.
SIMILARITY: Contains 1 peptidase S1 domain.
SIMILARITY: Contains 1 SRCR domain.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  MalaCards Disease Associations
  MalaCards Gene Search: TMPRSS2
Diseases sorted by gene-association score: prostatic acinar adenocarcinoma (16), prostate cancer 1 (12), influenza (12), prostate cancer (11), male reproductive organ cancer (9), prostate adenocarcinoma (9), severe acute respiratory syndrome (7), reproductive organ cancer (7), deafness, autosomal recessive 24 (6), small cell carcinoma (6), deafness, autosomal recessive 25 (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 85.73 RPKM in Prostate
Total median expression: 383.93 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -265.40516-0.514 Picture PostScript Text
3' UTR -642.901837-0.350 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR023415 - LDLR_class-A_CS
IPR002172 - LDrepeatLR_classA_rpt
IPR009003 - Pept_cys/ser_Trypsin-like
IPR018114 - Peptidase_S1/S6_AS
IPR001254 - Peptidase_S1_S6
IPR001314 - Peptidase_S1A
IPR001190 - Srcr_rcpt
IPR017448 - Srcr_rcpt-rel

Pfam Domains:
PF00530 - Scavenger receptor cysteine-rich domain
PF00089 - Trypsin

ModBase Predicted Comparative 3D Structure on O15393
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004252 serine-type endopeptidase activity
GO:0005044 scavenger receptor activity
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008236 serine-type peptidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0006508 proteolysis
GO:0006898 receptor-mediated endocytosis
GO:0016540 protein autoprocessing
GO:0046598 positive regulation of viral entry into host cell

Cellular Component:
GO:0005576 extracellular region
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0070062 extracellular exosome

-  Descriptions from all associated GenBank mRNAs
  BC051839 - Homo sapiens transmembrane protease, serine 2, mRNA (cDNA clone MGC:60144 IMAGE:6199625), complete cds.
AF329454 - Homo sapiens epitheliasin (TMPRSS2) mRNA, complete cds.
AF318374 - Homo sapiens pp9284 mRNA, complete cds.
AK123948 - Homo sapiens cDNA FLJ41954 fis, clone PROST2019296.
JD564945 - Sequence 545969 from Patent EP1572962.
JD146070 - Sequence 127094 from Patent EP1572962.
JD171853 - Sequence 152877 from Patent EP1572962.
JD500844 - Sequence 481868 from Patent EP1572962.
JD325528 - Sequence 306552 from Patent EP1572962.
JD109985 - Sequence 91009 from Patent EP1572962.
JD188326 - Sequence 169350 from Patent EP1572962.
JD514611 - Sequence 495635 from Patent EP1572962.
JD287946 - Sequence 268970 from Patent EP1572962.
JD245164 - Sequence 226188 from Patent EP1572962.
JD236884 - Sequence 217908 from Patent EP1572962.
JD154479 - Sequence 135503 from Patent EP1572962.
JD026382 - Sequence 7406 from Patent EP1572962.
JD036031 - Sequence 17055 from Patent EP1572962.
JD113837 - Sequence 94861 from Patent EP1572962.
JD050727 - Sequence 31751 from Patent EP1572962.
U75329 - Human serine protease mRNA, complete cds.
JD153848 - Sequence 134872 from Patent EP1572962.
JD034101 - Sequence 15125 from Patent EP1572962.
JD020701 - Sequence 1725 from Patent EP1572962.
JD551806 - Sequence 532830 from Patent EP1572962.
AK296860 - Homo sapiens cDNA FLJ56457 complete cds, highly similar to Transmembrane protease, serine 2 precursor (EC 3.4.21.-).
AK222784 - Homo sapiens mRNA for transmembrane protease, serine 2 variant, clone: HEP02362.
JD374260 - Sequence 355284 from Patent EP1572962.
JD539059 - Sequence 520083 from Patent EP1572962.
JD087352 - Sequence 68376 from Patent EP1572962.
JD409427 - Sequence 390451 from Patent EP1572962.
JD390875 - Sequence 371899 from Patent EP1572962.
JD156495 - Sequence 137519 from Patent EP1572962.
JD341878 - Sequence 322902 from Patent EP1572962.
AK291813 - Homo sapiens cDNA FLJ78182 complete cds, highly similar to Homo sapiens transmembrane protease, serine 2, mRNA.
AF270487 - Homo sapiens androgen-regulated serine protease TMPRSS2 precursor (TMPRSS2) mRNA, complete cds.
BC035623 - Homo sapiens transmembrane protease, serine 2, mRNA (cDNA clone IMAGE:5187060).
JD268538 - Sequence 249562 from Patent EP1572962.
HQ448630 - Synthetic construct Homo sapiens clone IMAGE:100072069; CCSB011816_03 transmembrane protease, serine 2 (TMPRSS2) gene, encodes complete protein.
KJ897688 - Synthetic construct Homo sapiens clone ccsbBroadEn_07082 TMPRSS2 gene, encodes complete protein.
AF123453 - Homo sapiens transmembrane serine protease 2 (TMPRSS2) mRNA, complete cds.
AK313338 - Homo sapiens cDNA, FLJ93860, highly similar to Homo sapiens transmembrane protease, serine 2 (TMPRSS2), mRNA.
CU690854 - Synthetic construct Homo sapiens gateway clone IMAGE:100021458 5' read TMPRSS2 mRNA.
BC015819 - Homo sapiens transmembrane protease, serine 2, mRNA (cDNA clone IMAGE:4273327), with apparent retained intron.
EU314931 - Homo sapiens TMPRSS2/ETV5 fusion isoform c mRNA, complete sequence.
EU314930 - Homo sapiens TMPRSS2/ETV5 fusion isoform b mRNA, complete sequence.
HM245389 - Homo sapiens TMPRSS2-FKBP5 breakpoint junction transcript variant 3 and FKBP5-ERG breakpoint junction transcript variant 3 mRNA sequence.
HM245390 - Homo sapiens TMPRSS2-FKBP5 breakpoint junction transcript variant 4 and FKBP5-ERG breakpoint junction transcript variant 4 mRNA sequence.
EU090249 - Homo sapiens TMPRSS2/ETV1 fusion mRNA, partial sequence.
AK092554 - Homo sapiens cDNA FLJ35235 fis, clone PROST2001599.
DQ204771 - Homo sapiens TMPRSS2/ETV1b fusion transcript.
DQ204770 - Homo sapiens TMPRSS2/ETV1a fusion transcript.
JD472095 - Sequence 453119 from Patent EP1572962.
JD076366 - Sequence 57390 from Patent EP1572962.
JD237007 - Sequence 218031 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: AF329454, B2R8E5, D3DSJ2, O15393, PRSS10, Q6GTK7, Q9BXX1, TMPS2_HUMAN, uc288tbi.1
UCSC ID: ENST00000678348.1
RefSeq Accession: NM_005656
Protein: O15393 (aka TMPS2_HUMAN or TMS2_HUMAN)
CCDS: CCDS33564.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.