RBD Mut Expr Track Settings
 
S RBD Deep Mutational Scanning: Expression (Jesse Bloom's Group)   (All Immunology tracks)

Display mode:       Reset to defaults

Type of graph:
Track height: pixels (range: 11 to 128)
Data view scaling: Always include zero: 
Vertical viewing range: min:  max:   (range: 0 to 127)
Transform function:Transform data points by: 
Windowing function: Smoothing window:  pixels
Negate values:
Draw y indicator lines:at y = 0.0:    at y =
Graph configuration help
All subtracks:
List subtracks: only selected/visible    all    ()  
hide
 Configure
 A_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 C_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 D_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 E_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 F_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 G_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 H_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 I_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 K_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 L_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 M_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 N_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 P_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 Q_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 R_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 S_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 T_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 V_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 W_expr_avg  DMS data for RBD expression   Data format 
hide
 Configure
 Y_expr_avg  DMS data for RBD expression   Data format 
    
Assembly: SARS-CoV-2 Jan. 2020 (NC_045512.2)

Description

This track contains deep mutational scanning data measuring the effect of the change in expression, ACE2 or antibody binding from a wild type allele to a mutant allele. The authors use a yeast display system to experimentally measure the effect of all possible point (amino acid) RBD mutations on protein expression, ACE2 affinity or antibody binding.

Display Conventions and Configuration

Each subtrack contains all the scores representing mutations to a particular amino acid (each annotation is a S codon). For instance the A subtrack measures the change in expression of S RBD expression if the annotated amino acid is mutated to alanine (if the wildtype amino acid is A, then the score is 0). A positive score indicates increased expression a negative score is a loss of expression.

Please see the interactive heatmap generated by the authors at this link. Structural visualizations of the data are available from the authors via dms-view here.

Methods

Table S2 from Starr et al, was downloaded and parsed into bedGraph format using the average value of both replicates reported. All NA values were filtered out.

Data Access

The raw data can be explored interactively with the Table Browser, or combined with other datasets in the Data Integrator tool.

Please refer to our mailing list archives for questions, or our Data Access FAQ for more information.

References

Starr TN, Greaney AJ, Hilton SK, Ellis D, Crawford KHD, Dingens AS, Navarro MJ, Bowen JE, Tortorici MA, Walls AC et al. Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding. Cell. 2020 Sep 3;182(5):1295-1310.e20. PMID: 32841599; PMC: PMC7418704